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Can I Love You and Hate You at the Same Time? - Are pain and anger in themselves toxic forces or violent acts, that negate the love we feel for those who are close to us? Does this uneasy sense of contradiction cause us to hold those 'negative' emotions inside ourselves? Tags: in practice, relationships...
Feed Source: counsellingresource.com

The Heart Sutra and the Neuroscientist - The neurosurgeon and the Buddhist practitioner are both intimate with the reality that between the atoms of the electrochemical dance of the brain lies an emptiness of anything at all which is solid and unchangeable, anything on which to lean. How to live in such a world? Tags: book, brain, meditation, mind-body, mindful awareness, ...
Feed Source: counsellingresource.com

Creative Regeneration - Bathing in my own attention, my breath, exercising the 'coming back' muscle whenever I get distracted, is what I call basic meditation. Every moment in this state is a moment of creative regeneration. Tags: anxiety and stress, meditation, mindful awareness, work-life...
Feed Source: counsellingresource.com

New Year and Its Pressures - At New Year there seems to be a lot of pressure -- to have a good time, to make New Year's eve a perfect night, and then to top it all with a fresh start in life. Does it have to be this way? Tags: anxiety and stress, meaning, mindful awareness...
Feed Source: counsellingresource.com

Going at Your Own Pace - When it feels like everything is speeding up around us, working against the tide and putting pressure on ourselves to force our own pace to match rarely offers the best way forward. Tags: anxiety and stress, mindful awareness...
Feed Source: counsellingresource.com

Tips for Living the Fully Conscious Life - Often, we're only half-listening or half-awake when we do things. That's the essence of life on autopilot. But there have been some truly remarkable souls among us who lived with greater than typical awareness. Tags: mindful awareness, presence...
Feed Source: counsellingresource.com

Not Moving the Place Where it Hurts - Instead of tackling a problem 'head on', what if we just avoid it? This sounds counter-intuitive, but I don't mean pretending the problem doesn't exist; quite the contrary. Tags: mindful awareness, therapy...
Feed Source: counsellingresource.com

The Inner Abuser - Why do we tolerate comments from ourselves, which we would not tolerate from anyone else? Indeed, why do we sometimes allow whole running commentaries on our way of being to take up every second of the day and a significant amount of our mental and emotional space? Tags: abuse and trauma, eating disorders, relationships...
Feed Source: counsellingresource.com

The Sudden Desire to End Therapy: Resistance or Wisdom? - Often when clients suddenly decide they want to end therapy, therapists are encouraged by the 'therapy culture' to think of it as some kind of resistance to the process. I wonder, is this necessarily the case? Tags: in practice, power, therapy...
Feed Source: counsellingresource.com

Dealing With ?Old? Feelings, and the ?Inner Child? Cringe - When you realise that overwhelming feelings can belong to you but not belong to you now or as the person you are today, it really is possible to soothe them in real time. Often these feelings belong to childhood, and turning to those child-feelings with gentleness is of huge benefit. So why does the term 'inner child' make me cringe? Tags: in practice, therapy...
Feed Source: counsellingresource.com

Genome-wide identification of interferon-sensitive mutations enables influenza vaccine design - In conventional attenuated viral vaccines, immunogenicity is often suboptimal. Here we present a systematic approach for vaccine development that eliminates interferon (IFN)–modulating functions genome-wide while maintaining virus replication fitness. We applied a quantitative high-throughput genomics system to influenza A virus that simultaneously measured the replication fitness and IFN sensitivity of mutations across the entire genome. ...
Feed Source: science.sciencemag.org

A self-assembled nanoscale robotic arm controlled by electric fields - The use of dynamic, self-assembled DNA nanostructures in the context of nanorobotics requires fast and reliable actuation mechanisms. We therefore created a 55-nanometer–by–55-nanometer DNA-based molecular platform with an integrated robotic arm of length 25 nanometers, which can be extended to more than 400 nanometers and actuated with externally applied electrical fields. Precise, computer-controlled switching of the arm between ar...
Feed Source: science.sciencemag.org

Quantum liquid droplets in a mixture of Bose-Einstein condensates - Quantum droplets are small clusters of atoms self-bound by the balance of attractive and repulsive forces. Here, we report on the observation of droplets solely stabilized by contact interactions in a mixture of two Bose-Einstein condensates. We demonstrate that they are several orders of magnitude more dilute than liquid helium by directly measuring their size and density via in situ imaging. We show that the droplets are stablized against coll...
Feed Source: science.sciencemag.org

Hydraulic fracturing volume is associated with induced earthquake productivity in the Duvernay play - A sharp increase in the frequency of earthquakes near Fox Creek, Alberta, began in December 2013 in response to hydraulic fracturing. Using a hydraulic fracturing database, we explore relationships between injection parameters and seismicity response. We show that induced earthquakes are associated with completions that used larger injection volumes (104 to 105 cubic meters) and that seismic productivity scales linearly with injection volume. In...
Feed Source: science.sciencemag.org

Chiromagnetic nanoparticles and gels - Chiral inorganic nanostructures have high circular dichroism, but real-time control of their optical activity has so far been achieved only by irreversible chemical changes. Field modulation is a far more desirable path to chiroptical devices. We hypothesized that magnetic field modulation can be attained for chiral nanostructures with large contributions of the magnetic transition dipole moments to polarization rotation. We found that dispersio...
Feed Source: science.sciencemag.org

Digitization of multistep organic synthesis in reactionware for on-demand pharmaceuticals - Chemical manufacturing is often done at large facilities that require a sizable capital investment and then produce key compounds for a finite period. We present an approach to the manufacturing of fine chemicals and pharmaceuticals in a self-contained plastic reactionware device. The device was designed and constructed by using a chemical to computer-automated design (ChemCAD) approach that enables the translation of traditional bench-scale syn...
Feed Source: science.sciencemag.org

A global atlas of the dominant bacteria found in soil - The immense diversity of soil bacterial communities has stymied efforts to characterize individual taxa and document their global distributions. We analyzed soils from 237 locations across six continents and found that only 2% of bacterial phylotypes (~500 phylotypes) consistently accounted for almost half of the soil bacterial communities worldwide. Despite the overwhelming diversity of bacterial communities, relatively few bacterial taxa are a...
Feed Source: science.sciencemag.org

Improving refugee integration through data-driven algorithmic assignment - Developed democracies are settling an increased number of refugees, many of whom face challenges integrating into host societies. We developed a flexible data-driven algorithm that assigns refugees across resettlement locations to improve integration outcomes. The algorithm uses a combination of supervised machine learning and optimal matching to discover and leverage synergies between refugee characteristics and resettlement sites. The algorith...
Feed Source: science.sciencemag.org

Dicer uses distinct modules for recognizing dsRNA termini - Invertebrates rely on Dicer to cleave viral double-stranded RNA (dsRNA), and Drosophila Dicer-2 distinguishes dsRNA substrates by their termini. Blunt termini promote processive cleavage, while 3' overhanging termini are cleaved distributively. To understand this discrimination, we used cryo–electron microscopy to solve structures of Drosophila Dicer-2 alone and in complex with blunt dsRNA. Whereas the Platform-PAZ domains have been consid...
Feed Source: science.sciencemag.org

Phosphoethanolamine cellulose: A naturally produced chemically modified cellulose - Cellulose is a major contributor to the chemical and mechanical properties of plants and assumes structural roles in bacterial communities termed biofilms. We find that Escherichia coli produces chemically modified cellulose that is required for extracellular matrix assembly and biofilm architecture. Solid-state nuclear magnetic resonance spectroscopy of the intact and insoluble material elucidates the zwitterionic phosphoethanolamine modificati...
Feed Source: science.sciencemag.org

Structural mechanisms of centromeric nucleosome recognition by the kinetochore protein CENP-N - Accurate chromosome segregation requires the proper assembly of kinetochore proteins. A key step in this process is the recognition of the histone H3 variant CENP-A in the centromeric nucleosome by the kinetochore protein CENP-N. We report cryo–electron microscopy (cryo-EM), biophysical, biochemical, and cell biological studies of the interaction between the CENP-A nucleosome and CENP-N. We show that human CENP-N confers binding specificit...
Feed Source: science.sciencemag.org

Multiplexed gene synthesis in emulsions for exploring protein functional landscapes - Improving our ability to construct and functionally characterize DNA sequences would broadly accelerate progress in biology. Here, we introduce DropSynth, a scalable, low-cost method to build thousands of defined gene-length constructs in a pooled (multiplexed) manner. DropSynth uses a library of barcoded beads that pull down the oligonucleotides necessary for a gene’s assembly, which are then processed and assembled in water-in-oil emulsi...
Feed Source: science.sciencemag.org

Toward dynamic structural biology: Two decades of single-molecule Förster resonance energy transfer - Classical structural biology can only provide static snapshots of biomacromolecules. Single-molecule Förster resonance energy transfer (smFRET) paved the way for studying dynamics in macromolecular structures under biologically relevant conditions. Since its first implementation in 1996, smFRET experiments have confirmed previously hypothesized mechanisms and provided new insights into many fundamental biological processes, such as DNA m...
Feed Source: science.sciencemag.org

Membrane protein insertion through a mitochondrial {beta}-barrel gate - The biogenesis of mitochondria, chloroplasts, and Gram-negative bacteria requires the insertion of β-barrel proteins into the outer membranes. Homologous Omp85 proteins are essential for membrane insertion of β-barrel precursors. It is unknown if precursors are threaded through the Omp85-channel interior and exit laterally or if they are translocated into the membrane at the Omp85-lipid interface. We have mapped the interaction of a pr...
Feed Source: science.sciencemag.org

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